Table I.
Saccharomyces cerevisiae strains used in this study
| Strain | Genotype | Source |
| FY406 | MATa his3Δ200 leu2Δ1 lys2-128Δ trp1Δ63 ura3-52 (hta1-htb1)Δ::LEU2 (hta2-htb2)Δ::TRP1, <pAB6 [URA3, HTA1-HTB1]> | Hirschhorn et al., 1995 |
| JDY183 | As FY406 except pJD150 [HTA1-HTB1, HIS3] replaces pAB6 | Downs et al., 2000 |
| JDY184 | As FY406 except pJD157 [HTA1-htb1-S125stop, HIS3] replaces pAB6 | This study |
| JDY5 | As FY406 except pJD149 [hta1-S129stop-HTB1] replaces pAB6 | This study |
| JDY6 | As FY406 except pJD167 [hta1-S129stop-htb1-S125stop] replaces pAB6 | This study |
| JDY7 | As FY406 except pJD168 [HTA1-htb1-T128stop, HIS3] replaces pAB6 | This study |
| JDY8 | As FY406 except pJD180 [hta1-S129stop-htb1-T128stop, HIS3] replaces pAB6 | This study |
| JDY185 | As FY406 except pJD172 [HTA1-htb1-S125A/S126A, HIS3] replaces pAB6 | This study |
| JDY186 | As FY406 except pJD173 [HTA1-htb1-K123R, HIS3] replaces pAB6 | This study |
| JDY187 | As FY406 except pJD174 [HTA1-htb1-K123R/S125A/S126A, HIS3] replaces pAB6 | This study |
| JDY630 | As FY406 except pJD183 [HTA1-Flag-HTB1, HIS3] replaces pAB6 | This study |
| JDY206 | As FY406 except pJD287 [HTA1-Flag-htb1-S125A/S126A, HIS3] replaces pAB6 | This study |
| JDY251 | As FY406 except pJD288 [HTA1-Flag-htb1-K123R, HIS3] replaces pAB6 | This study |
| JDY253 | As FY406 except pJD253 [HTA1-Flag-htb1-K123R/S125A/S126A, HIS3] replaces pAB6 | This study |
| Y131 | MATa ura3-1 leu2-3,-112 ade2-1 trp1-1 his3-11,15 can1-100 hta1-htb1::LEU2, hta2-htb2Δ <pRS426-HTA1-HTB1[URA3]> | Kao et al., 2004; Robzyk et al., 2000 |
| Y133 | MATa ura3-1 leu2-3,-112 ade2-1 trp1-1 his3-11,15 can1-100 hta1-htb1::LEU2, hta2-htb2Δ <pRS426-HTA1-htb1-K123R[URA3]> | Kao et al., 2004; Robzyk et al., 2000 |
| YM5037 | As Y131 except DOT1-13Myc::KANMX | Shahbazian et al., 2005 |
| YM5038 | As Y133 except DOT1-13Myc::KANMX | Shahbazian et al., 2005 |
| JDY631 | As YM5037 except pJD150 [HTA1-HTB1, HIS3] | This study |
| JDY632 | As YM5037 except pJD172 [HTA1-htb1-S125A/S126A, HIS3] | This study |
| JDY633 | As YM5037 except pJD173 [HTA1-htb1-K123R, HIS3] | This study |
| JDY634 | As YM5038 except pJD173 [HTA1-htb1-K123R, HIS3] | This study |
| JDY635 | As YM5037 except pJD174 [HTA1-htb1-K123R/S125A/S126A, HIS3] | This study |
| W303α | Matα ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 rad5-535 | R. Rothsteina |
| JDY286 | bre1Δ::KANMX in W303α | This study |
| SKY2849 | MATa ade2-1 can1-100 ura3-1 leu2-3,112 his3-11,15 trp1-1 dot1Δ::TRP1 | Wysocki et al., 2005 |
| SKY2856 | MATa ade2-1 can1-100 ura3-1 leu2-3,112 his3-11,15 trp1-1 set1Δ::HIS3MX6 | Wysocki et al., 2005 |
| SKY2859 | MATa ade2-1 can1-100 ura3-1 leu2-3,112 his3-11,15 trp1-1 dot1Δ::TRP1 set1Δ::HIS3MX6 | Wysocki et al., 2005 |
| JDY193 | bre1Δ::KANMX and <pJD150[HTA1-HTB1, HIS3]> in FY406 | This study |
| JDY195 | As JDY193 except <pJD172 [HTA1-htb1-S125A/S126A, HIS3]> | This study |
| JDY196 | As JDY193 except <pJD173 [HTA1-htb1-K123R, HIS3]> | This study |
| JDY197 | As JDY193 except <pJD174 [HTA1-htb1-K123R/S125A/S126A]> | This study |
| JDY771 | MATa/MATα ura3-1/ura3-1 leu2-3,-112/leu2-3,-112 ade2-1/ade2-1 trp1-1/trp1-1 his3-11,15/his3-11,15 can1-100/can1-100 DOT1-13Myc::KANMX/ DOT1-13Myc::KANMX hta1-htb1::LEU2/ hta1-htb1::LEU2, hta2-htb2Δ/hta2-htb2Δ <pRS426-HTA1-HTB1[URA3]> <pJD150 [HTA-HTB1, HIS3]> | This study |
| JDY772 | MATa/MATα ura3-1/ura3-1 leu2-3,-112/leu2-3,-112 ade2-1/ade2-1 trp1-1/trp1-1 his3-11,15/his3-11,15 can1-100/can1-100 DOT1-13Myc::KANMX/ DOT1-13Myc::KANMX hta1-htb1::LEU2/ hta1-htb1::LEU2, hta2-htb2Δ/hta2-htb2Δ <pRS426-HTA1-htb1-K123R[URA3]> <pJD150 [HTA1-HTB1, HIS3]> | This study |
a
Columbia University, New York, NY.