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. 2007 Oct 27;6(2):247–256. doi: 10.1093/ecam/nem080

Table 3.

Effects of NYT and IL-3 plus EPO on formation of CFU-E and BFU-E colonies in cultured marrow cells from mice treated with 5-FU

Day Treatment 5-FU Dose Cell viability CFU-E BFU-E mix n
(150 mg kg–1) (mg kg–1 day–1) (%) (×103 per femur) (×102 per femur)
5 Normal 95.7 ± 0.6 43.6 ± 1.0 32.0 ± 0.6 5
Control + 94.8 ± 0.1 1.6 ± 0.3# 0.9 ± 0.1# 9
NYT + 1 93.0 ± 0.1 2.2 ± 0.5 2.0 ± 0.9* 10
NYT + 10 94.5 ± 0.4 3.3 ± 0.5# 9.6 ± 1.2# 8
NYT + 100 97.6 ± 0.1 9.0 ± 0.7# 11.1 ± 0.5# 8
IL-3/EPO + 50 ng + 5 U 96.6 ± 0.3 18.6 ± 1.9# 15.5 ± 2.0# 8
10 Control + 93.7 ± 0.8 39.0 ± 2.1 8.0 ± 0.4# 7
NYT + 1 92.2 ± 1.0 37.9 ± 4.0 8.2 ± 0.1 8
NYT + 10 96.2 ± 0.4 45.2 ± 1.8# 8.8 ± 0.1 8
NYT + 100 94.4 ± 0.4 57.9 ± 3.3# 10.0 ± 0.3# 7
IL-3/EPO + 50 ng + 5 U 94.9 ± 0.6 81.9 ± 9.7# 13.8 ± 0.3# 7
15 Control + 97.4 ± 0.2 29.2 ± 1.1# 20.0 ± 1.4# 8
NYT + 1 97.5 ± 1.0 32.2 ± 3.0 31.2 ± 0.6# 8
NYT + 10 94.5 ± 0.7 40.0 ± 3.5# 36.8 ± 0.3# 9
NYT + 100 95.4 ± 0.6 57.9 ± 2.4# 51.7 ± 2.4# 8
IL-3/EPO + 50 ng + 5 U 95.1 ± 0.1 77.1 ± 4.7# 69.1 ± 1.3# 8

Mice were injected orally with NYT or subcutaneously with IL-3 (50 ng per mouse) and EPO (5 U per mouse) for 7 consecutive days before and after 5-FU injection. Marrow cells were collected at 5, 10 and 15 days after 5-FU injection, and cultured with 2 U ml–1 EPO with (for BFU-E mix) or without 5 ng ml–1 IL-3 (for CFU-E). The developing colonies were enumerated 2 (CFU-E) or 9 (BFU-E mix) days after cultivation. Data are expressed as the mean ± SE.

#P < 0.05 compared to normal mice. *P < 0.05 compared to 5-FU-treated mice.