Table 3.
Effects of NYT and IL-3 plus EPO on formation of CFU-E and BFU-E colonies in cultured marrow cells from mice treated with 5-FU
| Day | Treatment | 5-FU | Dose | Cell viability | CFU-E | BFU-E mix | n |
|---|---|---|---|---|---|---|---|
| (150 mg kg–1) | (mg kg–1 day–1) | (%) | (×103 per femur) | (×102 per femur) | |||
| 5 | Normal | – | – | 95.7 ± 0.6 | 43.6 ± 1.0 | 32.0 ± 0.6 | 5 |
| Control | + | – | 94.8 ± 0.1 | 1.6 ± 0.3# | 0.9 ± 0.1# | 9 | |
| NYT | + | 1 | 93.0 ± 0.1 | 2.2 ± 0.5 | 2.0 ± 0.9* | 10 | |
| NYT | + | 10 | 94.5 ± 0.4 | 3.3 ± 0.5# | 9.6 ± 1.2# | 8 | |
| NYT | + | 100 | 97.6 ± 0.1 | 9.0 ± 0.7# | 11.1 ± 0.5# | 8 | |
| IL-3/EPO | + | 50 ng + 5 U | 96.6 ± 0.3 | 18.6 ± 1.9# | 15.5 ± 2.0# | 8 | |
| 10 | Control | + | – | 93.7 ± 0.8 | 39.0 ± 2.1 | 8.0 ± 0.4# | 7 |
| NYT | + | 1 | 92.2 ± 1.0 | 37.9 ± 4.0 | 8.2 ± 0.1 | 8 | |
| NYT | + | 10 | 96.2 ± 0.4 | 45.2 ± 1.8# | 8.8 ± 0.1 | 8 | |
| NYT | + | 100 | 94.4 ± 0.4 | 57.9 ± 3.3# | 10.0 ± 0.3# | 7 | |
| IL-3/EPO | + | 50 ng + 5 U | 94.9 ± 0.6 | 81.9 ± 9.7# | 13.8 ± 0.3# | 7 | |
| 15 | Control | + | – | 97.4 ± 0.2 | 29.2 ± 1.1# | 20.0 ± 1.4# | 8 |
| NYT | + | 1 | 97.5 ± 1.0 | 32.2 ± 3.0 | 31.2 ± 0.6# | 8 | |
| NYT | + | 10 | 94.5 ± 0.7 | 40.0 ± 3.5# | 36.8 ± 0.3# | 9 | |
| NYT | + | 100 | 95.4 ± 0.6 | 57.9 ± 2.4# | 51.7 ± 2.4# | 8 | |
| IL-3/EPO | + | 50 ng + 5 U | 95.1 ± 0.1 | 77.1 ± 4.7# | 69.1 ± 1.3# | 8 |
Mice were injected orally with NYT or subcutaneously with IL-3 (50 ng per mouse) and EPO (5 U per mouse) for 7 consecutive days before and after 5-FU injection. Marrow cells were collected at 5, 10 and 15 days after 5-FU injection, and cultured with 2 U ml–1 EPO with (for BFU-E mix) or without 5 ng ml–1 IL-3 (for CFU-E). The developing colonies were enumerated 2 (CFU-E) or 9 (BFU-E mix) days after cultivation. Data are expressed as the mean ± SE.
#P < 0.05 compared to normal mice. *P < 0.05 compared to 5-FU-treated mice.