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CA125 levels in cell lysates and conditioned medium
| Cell lines | CA 125, units/ml
|
|
|---|---|---|
| Cell lysates | Cell medium | |
| 3T3 (negative) | <4 | <4 |
| OVCAR-3 (positive) | 60,000 | 7,000 |
| IOSE-29 | <4 | 41 |
| IOSE-29EC | 82,000 | 6,000 |
Cells were grown to subconfluence and lysed (cell lysates), or the medium was changed and collected 1 day later (culture medium). One hundred micrograms of cell lysate and 300 μg of medium then were assayed for CA125 by microparticle enzyme immunoassay.
