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Effect of LH on expression of 11β-HSD2 protein. R2C cells were transfected with pcDNA3.0 (lane 1) or pcDNA-hsd11b2 (lane 2); Leydig cells were treated with vehicle (PBS for LH, lane 3) or 20 ng/ml LH for 24 h (lane 4). At end of treatment, levels of 11β-HSD2 protein were determined by western blot analysis as described in Methods.
