Table 1.
Summary of cDNA fragments identified by the DD-PCR screen of ventral and dorsal midbrain regions.
| Clone ID |
Gene Bank accession number |
Gene product | Function | Expression |
| M5v3 | XM_001236352.1 | Kinase (PRKA) anchor protein 8-like (AKAP8L) | Cell division | n.d. |
| M12v2 | NM_001031296.1 | Splicing factor, arginine/serine-rich 11 SFRS11 | RNA processing | n.d. |
| F1d1 | NW_001471710 | SRP14 | RNA processing | Verified by RT-PCR |
| F0d1a | XM_414248.2 | Nucleolar RNA host gene 8 |
Nucleolar RNA | n.d |
| F0d4 | XM_001234485 | SMARCA5 | Chromatin modification | n.d. |
| M5v1 | NW_001471669 | Estrogen receptor α | Transcription | Dorso-ventral gradient |
| M0d1a | ref/NC_026897.1 | 16S ribosomal RNA | RNA metabolism | n.d. |
| M6v2 | XM_414131 | CCDC123 | Mitochondrial protein | n.d. |
| M17d2 | XM_415003.2 | Mitochondrial ribosomal protein S5 | Mitochondrial protein | n.d. |
| M4v1 | NM_001030819 | ABCC4 (CFTR/MRP) | Chloride channel | n.d. |
| M5v4, M12v3 | NW_001471710 | MEKKK5 | Signalling | Dorso-ventral gradient |
| M7v1 | NW_001471526 | AAT1-α | Signalling | Dorso-ventral gradient |
| M13v1a | XR_026877.1 | Sulfatase 1 SULF 1 | Signalling | n.d. |
| M4d2 | NW_001471434 | Naked cuticle (NKD1) homologue 1 | Signalling | Verified by RT-PCR |
| F0d3 | NW_001471529 | Similar to EAT2 | Signalling | Verified by RT-PCR |
| F4d1 | emb/AJ720092 | YWHAH | Signalling | n.d. |
| M5v2 | XM_419938 | KIAA0161, ring finger protein 144A (RNF144a) | Protein turnover | n.d. |
| M11d1 | NW_001471685 | QR1 (SPARC-like 1, Hevin) | Cell adhesion | Medio-dorsal |
| M5v6 | NW_001471633 | TEM7R/Plxdc2 | Migration | Verified by RT-PCR |
| F0d1, F0d2 | NW_001471503 | Tenascin C | ECM | Verified by RT-PCR |
| M1v1 | XM_426638 | Ferric-chelate reductase 1 | Iron metabolism | n.d. |
| M12d2 | NM_001012604. | Macrophage erythroblast attacher MAEA | Migration | n.d |
| M1v1a | ref/XR_026897.1 | Similar to MLL5 | Chromatin modifications | n.d. |
| F4v1 | XM_415718.2 | RNF43 | Protein turnover | n.d. |
The table itemizes the BLAST accession numbers, the chick nomenclature, the function and expression of the identified clones. The code of the clone IDs is as follows: M and F followed by the number stand for the primer combination used to make cDNA, v = ventral, d = dorsal. Abbreviations used: ECM = extracellular matrix, n.d. = not determined.