Table 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype or Descriptiona | Reference |
|---|---|---|
| Strains | ||
| C. violaceum CV026 | White indicator strain; cviI::Tn5 xylE; Ampr, Kanr, Strr, Tets, Erys, Chls | 27 |
| E. coli CA027ZC09 | The genomic clone generated from Ralstonia solanacearum GMI1000 for sequencing harbor plasmid pZC09 containing aac gene (RSc2547); Ampr | INRA-CNRSb |
| E. coli DH10B | F-mcrAΔ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 deoR recA1 endA1 araΔ139 Δ(ara leu)7697 galU galK λ-rpsL nupG; Strr | Invitrogen |
| E. coli BL21(DE3) | hsdS gal (λcIts857 ind1 Sam7 nin5 lacUV5-T7 gene 1) | Novagen |
| Candida tropicalis F-129 | Test strain for the MIC of aculeacin A assay | 29 |
| Plasmids | ||
| pZC09 | Plasmid generated from Ralstonia solanacearum GMI1000 for sequencing project from which the aac gene was amplified; Ampr | INRA-CNRSb |
| pBBR1MCS-3 | Mobilisable broad-host-range cloning vector; low copy number; mol, rep, lacZ; Tetr | 30 |
| pS3aac | Transcriptional fusion of aac gene in pBBR1MCS-3; Tetr | This study |
| pET21a | Expression vector; T7 promoter; C-terminal HisTag; lacI; Ampr | Novagen |
| pET21aac | Translational fusion of aac gene in pET21a; Ampr | This study |
a Amp: ampicillin; Kan: kanamycin; Tet: tetracycline; Nal: nalidixic acid; Str:
streptomycin; Chl: chloramphenicol; Ery: erythomycin
b INRA-CNRS: Laboratoire de Biologie Moleculaire des Relations Plantes
Microorganismes INRA-CNRS, France