Fig. 2.

Targeted CCN2 siRNA suppresses accumulation of αSMA or collagen deposition in preventative fibrosis model. Livers were sectioned and processed to detect αSMA (upper panel; brown stain) or collagen deposition (lower panel; blue stain) after administration of oil a or CCl₄ for 3 weeks b-e, the latter 2 weeks of which were given concurrent with either no therapy b, scrambled CCN2 siRNA in targeted liposomes c, CCN2 siRNA in non-targeted liposomes d, or CCN2 siRNA in targeted liposomes (E). CCl₄ was given i.m. as four 30 μl doses (1:1 CCl₄ : olive oil) per week. siRNA treatments (0.1 mg/kg) were given 4 times a week by i.p. injection. The staining intensity was assessed by image analysis (50 sections; 10 sections from each of 5 mice) and is shown in the bar graph. Data shown are indicative of the data from 3 separate experiments