Fig. 4.
Expression of fibrosis-related genes. Livers were removed from mice after administration of oil a or CCl4b-e for 3 weeks (preventative model; upper panel) or 5 weeks (curative model; lower panel). For the last 2 weeks in each model mice received no therapy b, scrambled CCN2 siRNA in targeted liposomes c, CCN2 siRNA in non-targeted liposomes d or CCN2 siRNA in targeted liposomes e. RNA was isolated and subjected to RT-PCR to establish the expression level of the indicated fibrotic marker genes as described (Lawrencia et al. 2009). Data are plotted relative to the expression of β-actin