Fig. 2.

CTGF/CCN2 expression is regulated by dexamethasone in HC11 cells. HC11 mammary epithelial cells were grown to confluence and stimulated with dexamethasone (DEX)(1 μM) in serum-containing media in the presence of insulin. RU486 at varying concentrations or vehicle (ethanol) was added to cells alone or in combination with dexamethasone. RNA was isolated and levels of CTGF and actin RNA were determined by realtime PCR. The results indicate the induction of CTGF/CCN2 normalized to actin. *These Dex+Ru486 values represent statistically significant difference (p-value .001) from the Dex alone condition