Table 1.
Elevation of gene expression following stimulation of HC11 cells with DIP
| mean of log ratio | GB acc | Description |
|---|---|---|
| 17.067 | NM_010217.1 | connective tissue growth factor |
| 13.447 | NM_138314.1 | protein expressed in non-metastatic cells 7 |
| 11.15 | XM_150141.2 | LOC234574 |
| 9.52 | NM_009263.1 | secreted phosphoprotein 1 |
| 7.69 | BC009155.1 | microsomal glutathione S-transferase 1 |
| 7.64 | NM_022032.1 | p53 apoptosis effector related to Pmp22 |
| 6.99 | NM_011326.1 | sodium channel, nonvoltage-gated 1 gamma |
| 6.44 | NM_009681.1 | adaptor-related protein complex AP-3, sigma 1 subunit |
| 6.34 | NM_029083.1 | RIKEN cDNA 5830413E08 gene |
| 5.92 | BC036990.1 | metallothionein 1 |
| 5.85 | XM_123496.1 | Tcfcp2-related transcriptional repressor 1 |
| 5.78 | NM_007621.1 | carbonyl reductase 2 |
| 5.77 | NM_138578.1 | Bcl2-like |
| 5.76 | NM_012519.1 | calcium/calmodulin-dependent protein kinase II, delta |
| 5.72 | NM_009976.1 | cystatin C |
| 5.42 | NM_010286.1 | glucocorticoid-induced leucine zipper |
| 5.15 | NM_009128.1 | stearoyl-Coenzyme A desaturase 2 |
| 5.14 | NM_025610.1 | RIKEN cDNA 2410004D18 gene |
| 5.13 | NM_011361.1 | serum/glucocorticoid regulated kinase |
| 4.88 | AK009928.1 | RIKEN cDNA 2310051E17 gene |
| 4.82 | NM_011313.1 | S100 calcium binding protein A6 (calcyclin) |
| 4.79 | AF434663.1 | immunoglobulin superfamily, member 4 |
| 4.75 | NM_010730.1 | annexin A1 |
| 4.60 | NM_026217.1 | autophagy 12-like (S. cerevisiae) |
| 4.60 | NM_025436.1 | sterol-C4-methyl oxidase-like |
| 4.52 | NM_010884.1 | N-myc downstream regulated 1 |
| 4.48 | XM_177182.1 | expressed sequence C85344 |
| 4.44 | NM_011400.1 | solute carrier family 2 (facilitated glucose transporter) |
| 4.41 | NM_011803.1 | core promoter element binding protein |
| 4.41 | NM_010907.1 | nuclear factor of kappa light chain gene enhancer |
| 4.34 | NM_013470.1 | annexin A3 |
| 4.33 | NM_145942.1 | 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1 |
| 4.21 | AL080093.1 | expressed sequence AU067636 |
| 4.16 | XM_132485.1 | RAS-related C3 botulinum substrate 1 |
| 4.11 | NM_010762.1 | myelin and lymphocyte protein, T-cell differentiation |
| 4.10 | NM_009983.2 | cathepsin D |
| 4.08 | AF378830.1 | prostaglandin-endoperoxide synthase 2 |
| 4.05 | NM_145977.1 | RIKEN cDNA 2210413P12 gene |
| 4.01 | NM_146120.1 | gelsolin |
| 3.98 | BC020081.1 | RIKEN cDNA 2310016C08 gene |
| 3.95 | BC019207.1 | low density lipoprotein receptor |
| 3.95 | NM_053623.1 | expressed sequence AU018108 |
| 3.93 | NM_019971.1 | platelet-derived growth factor, C polypeptide |
| 3.92 | NM_013492.1 | clusterin |
| 3.89 | NM_008084.1 | glyceraldehyde-3-phosphate dehydrogenase |
| 3.87 | BC038392.1 | ATPase, H+ transporting, lysosomal 70kD, V1 |
| 3.85 | NM_033079.1 | DNA segment, Chr 6, Miriam Meisler 5, expressed |
| 3.84 | BC037116.1 | adducin 3 (gamma) |
Regulation of gene expression in HC11 cells undergoing lactogenic differentiation. To determine the effect of lactogenic differentiation on gene transcription in HC11 cells oligonucleotide microarrays representing 20280 genes (NIA 20 k arrays from Agilent Technologies) were hybridized with RNA from DIP treated HC11 cells. The RNA was labeled with cyanine 5-CTP and control RNA was labeled with cyanine 3-CTP. Fluorescent cRNA was synthesized and hybridized in the differentiation experiments (N = 5) as described in Materials and Methods. Analysis of gene expression was performed using BRB-Array Tools Version 3. In The HC11-DIP versus HC11-control RNA experiments, there are 10813 genes that passed filtering criteria in total of 20280 genes. The first 2479 genes were significant at the nominal 0.05 level of the paired T-test, and 998 genes met the 2-fold change criteria. Table 1 shows the first 50 genes with elevated expression in cells undergoing lactogenic differentiation