Table 1.

Effect of selected protein kinase inhibitors on ATP-mediated release of IL-1β from LPS-primed monocytes^a

Stimulator/inhibitor	Concentration of inhibitors	% Inhibition
LPS + ATP		0
Me2SO	0.01%	0
Genistein	50 μM	7
AG1296	1.0 μM	0
AG1478	10.0 μM	4
AG126	50.0 μM	88
PPI	1.0 μM	0
Herbimycin A	1.0 μg/ml	35

^aAdapted from [5]. Cells stimulated with 1 ng/ml of LPS for 3 h, treated with the inhibitors for 30 min, and then stimulated with ATP 5 mM for 30 min. Supernatants harvested and assayed for IL-1β. Date is percent inhibition relative to LPS + ATP