Fig. 4.

TUNEL staining in the immediate-treatment group. TUNEL-positive cells were evident both in WM such as the optic tract (A) and internal capsule (B) and also in gray matter such as the infarcted cortex (C) and subventricular zone (D). We only counted positive cells that were also stained with DAPI (E). TUNEL-positive cells in the immediate-treatment (F) and delayed-treatment (G) groups. The number of TUNEL-positive cells was lower for citicoline treatment than for PBS treatment in the immediate-treatment group, whilst there was no significant difference in the delayed-treatment group. Scale bars in A-E=200 µm. ^*p<0.05, ^**p<0.001. TUNEL: the terminal deoxynucleotidyl transferase biotin-dUTP nick end labelling, WM: white matter.