Figure 3.

SLPI does not inhibit augmented release of TNFα (A) nor IL-1β (B) from coincubation of stimulated THP-1 cells and human neutrophils. Representative data are shown by using neutrophils isolated from a single donor (BG). The experimental design was as described in Figs. 1 and 2 except that inhibitors were preincubated with cells for 15 min at 37°C before the addition of LPS and FNLP. SLPI was added at a final concentration of 2.5 μM. Levels of TNFα and IL-1β in the presence of SLPI alone and of SLPI plus CE-2072 were not measured for neutrophils nor for THP-1cells cultured separately.