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Effect of nicotine on the motility (A) and invasiveness (B) of the esophageal squamous carcinoma cell line TE-13. Numbers of TE-13 cells which migrated through the polycarbonate (A) or matrigel (B) membrane of Transwell® to the lower surface of the membrane were counted under a light microscope (× 400). Columns, mean of three triplicate experiments; bars, standard error of mean. bP < 0.01 vs the control group; dP < 0.01 vs the nicotine 100 mg/mL group.
