Figure 1.

Expression of FGF-1, FGF-7, and FGF-10 and their receptor FGFR2b during pancreas development. (A) Reverse transcriptase–PCR was carried out on pancreatic cDNAs from the indicated stages of development by using appropriate primers for each FGF. Thirty-five cycles of amplification were used for the amplification of FGFs transcripts. Cyclophilin amplification (30 cycles) was used to control the quality of the cDNAs. (B) Expression of FGFR2b mRNA in the developing rat pancreas. FGFR2b expression was analyzed by Northern blot. Total RNA from pancreases at different stages of development were hybridized successively with FGFR2b and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) probes.