Figure 2. Sns and Duf are expressed in Drosophila nephrocytes.

a-h, Sns (a,d,f,h) and Duf (b,c,e,g,h) expression in garland (a,b,d,e) and pericardial (c,f,g,h) nephrocytes. Embryonic (a,b, arrowheads), first instar larva (c, green, arrowheads), and third instar larvae (d-h, green). The actin cytoskeleton has been counterstained in c,d,f,g (red). h, Sns (h, green) and Duf (hⁱ, red) co-localise (hⁱⁱ, yellow). i-l, Clusters of ~6-8 wild-type (i,k), sns (j) or duf,rst (l) embryonic garland cells stained with anti-Duf (i,j) or anti-Sns (k,l). m-p, wild-type (m,o), sns-RNAi (n) and duf (p) third instar garland cells stained for anti-Duf (red) and anti-Sns (green) (merge appears yellow) and DNA (blue). Single optical section (m,n) or z-projection of cell surface (o,p) are shown. q-u, TEMs of wild-type third instar garland cells immunogold-stained for anti-Sns (q), anti-Duf (r,s) or double labelled (t,u). For double labelling 5nm (arrowhead) and 10nm (arrow) gold particles are used for Sns and Duf respectively. Scale bars 100nm (q-t) and 10nm (u).