Figure 2.

β-Trcp forms a complex with both β-catenin and Skp1. (A) Schematic diagram of wild-type β-Trcp, β-TrcpΔF, and β-TrcpΔWD. (B) Immunoprecipitation of β-catenin. The precipitates were examined for β-Trcp (Upper) and Skp1 (Lower). A longer exposure revealed that endogenous β-catenin also precipitated β-Trcp^myc (data not shown). (C) Immunoprecipitation of β-Trcp^myc using the same embryo extracts as in B. The precipitates were examined for Skp1 (Upper) and β-Trcp (Lower). (D) Immunoblot of Skp1 in the same embryo extracts as in B. RNA injected per embryo: 1 ng each for β-Trcp^myc and for deletion mutants β-catenin and Skp1.