TABLE 1.
Strains used in this study
| Strain | Description | Source or reference |
|---|---|---|
| S. pneumoniae | ||
| CP1250 | Rx derivative; low β-galactosidase activity; hex malM511 str-1 bgl-1; Hex− Mal−-Smr Bga− | 25 |
| CP1343 | CP1250, but ΔclpL::PcEm; Emr | This study |
| CP1344 | CP1250, but ΔclpC::PcTet; Tcr | This study |
| CP1359 | CP1250, but ΔclpP::PcTet; Tcr | This study |
| CP1360 | CP1250, but ΔclpE::PcKan; Kanr | This study |
| CP1361 | CP1250, but ΔclpX::PcEm slx; Emr | This study |
| CP1376 | CP1250, but ΔcomW::KANT; Kanr | 32 |
| CP1500 | Rx derivative; hex novo-r byra-r str-r1 ery-rG ery-r2; Novr Emr Strr | 6 |
| CP1851 | CP1250, but ΔclpE::PcEm; Emr | 32 |
| CP1890 | CPM7, but ΔclpP::PcTet; Tcr Cmr | CPM7 × CP1359a |
| CPM7 | CP1250, but ssbB::pEVP3::ssbB+; SsbB+ Cmr | 18 |
| E. coli | ||
| BL21(DE3) | F−ompT hsdSB (rB− mB−) gal dcm (DE3) | Invitrogen |
a
Recipient × donor; see Materials and Methods.