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. 2009 Mar 13;191(10):3237–3247. doi: 10.1128/JB.01837-08

FIG. 4.

FIG. 4.

Sortase C localizes to single foci in E. faecalis. (A) Piliation levels of wild-type OG1X and sortase mutants. Results are from a representative experiment in which ≥100 cells/strain/experiment were counted. Statistical significance measured by chi-square test: *, P < 0.001. **, P < 0.0001. (B) Coimmunolocalization of SecA (large particles) and SrtC (small particles) found together in foci. Inset, close-up of colocalized SecA and SrtC. Scale bar, 0.5 μm. (C) Quantitative analysis of SrtC immunoelectron micrographs. *, P < 0.0001 by chi-square test. (D) Location of SrtC foci in bacteria equally divided into three regions from youngest visible septum to pole. Statistical significance measured by Fisher's exact test: *, P < 0.05; **, P < 0.001.