FIG. 6.

Rescue of strain IB483 diamide sensitivity phenotype by plasmid ptrxD. Diamide disk diffusion assays were used to compare sensitivities of the wild-type strain 638R, the quadruple trx mutant strain IB498, the quintuple trx mutant strain IB483, and IB483 expressing trxD on plasmid ptrxD. Black bars represent plates placed directly into an anaerobic incubator after plating, and open bars represent plates placed in an aerobic incubator for 6 h prior to being placed into the anaerobic incubator. The values are mean diameters of growth inhibition from three independent experiments performed in triplicate, and are given in millimeters. The error bars indicate standard deviations. *, P < 0.001 compared to wild-type strain.