TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Phenotype and/or genotypea | Reference or source |
|---|---|---|
| B. fragilis strains | ||
| 638R | Clinical isolate, Rifr | 32 |
| ADB77 | 638R ΔthyA Rifr Tpr | 3 |
| IB298 | 638R ΔoxyR::tetQ Tetr Rifr | 37 |
| IB458 | ADB77 reverted to thyA+, ΔtrxC Rifr | This study |
| IB469 | ADB77 reverted to thyA+, ΔtrxD Rifr | This study |
| IB471 | ADB77 reverted to thyA+, ΔtrxG Rifr | This study |
| IB473 | ADB77 ΔthyA, ΔtrxA single crossover integrated into trxA gene, Rifr Tetr | This study |
| IB483 | ADB77 reverted to thyA+, ΔtrxC ΔtrxD::cfxA ΔtrxE ΔtrxF ΔtrxG Rifr Cfxr | This study |
| IB490 | ADB77 reverted to thyA+, ΔtrxE Rifr | This study |
| IB491 | ADB77 reverted to thyA+, ΔtrxF Rifr | This study |
| IB492 | ADB77 reverted to thyA+, ΔtrxE ΔtrxF Rifr | This study |
| IB498 | ADB77 reverted to thyA+, ΔtrxC ΔtrxE ΔtrxF ΔtrxG Rifr | This study |
| IB499 | IB498 ΔoxyR::tetQ Rifr Tetr | This study |
| IB500 | IB483 ΔoxyR::tetQ Rifr Cfxr Tetr | This study |
| E. coli strains | ||
| DH10B | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 deoR recA1 endA1 araD139 Δ(ara leu)7697 galU galK λ−rpsL nupG tonA | Invitrogen |
| HB101::RK231 | HB101 containing RK231, Kmr Tcr Str | 12 |
| Plasmids | ||
| pFD340 | Bacteroides-E. coli expression shuttle vector, (Ampr), Ermr | 44 |
| pFD516 | Bacteroides suicide vector, derived by deletion of Bacteroides replicon pBI143 from pFD288, (Spr), Ermr | 45 |
a
Ermr, erythromycin resistance; Cfxr, cefoxitin resistance; Rifr, rifampin resistance; Tetr, tetracycline resistance; Tpr, trimethoprim Spr, spectinomycin resistance; Ampr, ampicillin resistance. For Bacteroides-E. coli shuttle vectors, parentheses indicate antibiotic resistance expression in E. coli.