TABLE 2.
Plasmid | No. (%) of trxA deletions observed/total no. tested |
---|---|
None | 0/468 |
ptrxA | 27/84 (32) |
ptrxC | 0/23 |
ptrxD | 0/20 |
ptrxE | 0/51 |
ptrxF | 0/47 |
ptrxG | 0/72 |
Strain IB473 containing the trxA deletion construct single crossover integrated into the trxA gene was the recipient for all trx-containing plasmids. Deletions were identified by PCR analysis of the genomic DNA isolated from individual colonies that had resolved the trxA deletion construct (see Materials and Methods for more details).