FIG. 4.

Model of interactions among the insoluble spore coat proteins. Only proteins localized in the outer coat (OC) are indicated. Capital letters refer to appropriate Cot proteins. Schematic sections of the outer coat and inner coat (IC) are shaded. The direct contacts between proteins revealed by yeast two-hybrid assay are indicated by the direct contacts of the blocks representing individual Cot proteins. Dashed arrows show the assembly dependencies of one protein on another. In the case of CotE/CotZ, the dotted arrow shows some affinity between these proteins observed in the pull-down assay. In the case of CotE/CotH, the dotted arrow refers to an effect of CotH on CotE deposition (11).