TABLE 1.
Oligonucleotides used in this study
| Primer | Sequencea |
|---|---|
| CotE5′ | AGGAGGAGGCATATGTCTGAATACAGGGAAATTAT |
| CotE3′ | TGGTGGTGGGAATTCTTATTCTTCAGGATCTCCC |
| CotY5′ | AGGAGGAGGCATATGAGCTGCGGAAAAACCC |
| CotY3′ | AGGAGGAGGGAATTCTTATCCATTGTGATGATGCTTTTTATC |
| CotW5′ | AGGAGGAGGCATATGTCAGATAACGATAAATTCAAAG |
| CotW3′ | GGAGGAGGAGAATTCCTAATTGTTATCGTTTTTCCGTC |
| CotY5′Duet | TGGTGGTGGCATATGAGCTGCGGAAAAACCCATG |
| CotY3′Duet | TGGTGGTGGCTCGAGTTATCCATTGTGATGATGCTTTTTATC |
| CotZ5′Duet | TGGTGGTGGGAATTCAAGCCAGAAAACATCAAGCTG |
| CotZ3′Duet | TGGTGGTGGGTCGACTTAATGATGATGTGTACGATTG |
| CotV5′Duet | GATGATGATGGATCCTTCATTTGAAGAAAAAGTCGAATCC |
| CotV3′Duet | ATGATGATGCTGCAGTTAAAGGACGTCAAGTTCACTAAG |
| CotW3′Duet | GGAGGAGGACTCGAGCTAATTGTTATCGTTTTTCCGTC |
| Y2HcotE5′ | GGGGACAAGTTTGTACAAAAAAGCAGGCTTCTCTGAATACAGGGAAATTATTACGAAGG |
| Y2HcotE3b′ | GGGGACCACTTTGTACAAGAAAGCTGGGTCTCATTCTTCAGGATCTCCCACTAAAAAC |
| Y2HcotX5′ | GGGGACAAGTTTGTACAAAAAAGCAGGCTTCGAAAGCAGACCATATTCTTGGG |
| Y2HcotX3′ | GGGGACCACTTTGTACAAGAAAGCTGGGTCTCAGAGGACAAGAGTGATAACTAGG |
| Y2HcotY5′ | GGGGACAAGTTTGTACAAAAAAGCAGGCTTCAGCTGCGGAAAAACCCATGG |
| Y2HcotY3′ | GGGGACCACTTTGTACAAGAAAGCTGGGTCTCATCCATTGTGATGATGCTTTTTATCTTTG |
| Y2HcotZ5′ | GGGGACAAGTTTGTACAAAAAAGCAGGCTTCAGCCAGAAAACATCAAGCTGCGTG |
| Y2HcotZ3′ | GGGGACCACTTTGTACAAGAAAGCTGGGTCTTAATGATGATGTGTACGATTGATTAATCGAG |
| Y2HcotV5′ | GGGGACAAGTTTGTACAAAAAAGCAGGCTTCTCATTTGAAGAAAAAGTCGAATCCCTG |
| Y2HcotV3′ | GGGGACCACTTTGTACAAGAAAGCTGGGTCTTAAAGGACGTCAAGTTCACTAAGAATG |
| Y2HcotW5′ | GGGGACAAGTTTGTACAAAAAAGCAGGCTTCTCAGATAACGATAAATTCAAAGAAGAGC |
| Y2HcotW3′ | GGGGACCACTTTGTACAAGAAAGCTGGGTCTTAATTGTTATCGTTTTTCCGTCTTTGTC |
a
Restriction sites and attB1/attB2 sequences used for Gateway system cloning are underlined.