TABLE 2.
Interaction of spore coat proteins in the yeast two-hybrid system
| Activation domain | Protein activity in binding domaina
|
|||||
|---|---|---|---|---|---|---|
| CotE | CotV | CotW | CotX | CotY | CotZ | |
| CotE | +++ | − | − | − | − | − |
| CotV | − | − | + | − | − | − |
| CotW | − | + | − | − | − | − |
| CotX | − | − | − | − | − | − |
| CotY | − | − | − | − | +++ | +++ |
| CotZ | − | − | − | − | ++ | − |
Quantitative estimate of interactions was made based on the growth profiles on selection plates and the X-Gal assay. +++, strong interactions when the yeast colonies grow well on plates lacking histidine and uracil, do not grow in the presence of 5FOA, and an intensive blue color in the filter assay develops in <1 h; ++, moderately strong interactions when the yeast colonies grow well on plates lacking histidine and slightly less on plates lacking uracil, do not grow in the presence of 5FOA, and an intensive blue color in filter assay develops in <6 h; +, weak interactions when the yeast colonies grow slightly less than the control representing the strong interaction (CotE/CotE) on plates lacking histidine, do not grow on plates lacking uracil, grow slightly slower in the presence of 5FOA, and a light blue color in the filter assay develops overnight.