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. 2009 Mar 20;191(10):3339–3349. doi: 10.1128/JB.01782-08

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FIG. 4. — Accessibility of the sulfhydryl group of cysteine in the SlpA mutant proteins modified. Monomeric proteins in solution modified with PEG-maleimide for the 5-min reaction (A) and AlexaFluor488-maleimide for the 30-s reaction (B). (C) Proteins assembled on cell wall fragments modified with PEG-maleimide (5-min reaction). Bars indicate the proportion of modified protein from total protein (A and C) or the intensity of the fluorescence marker signal compared with the highest signal observed (B).