TABLE 2.
Analysis of pAM373 and cytolysin regions
| Region and strain | Cytolysin activity | Integration (PCR) | CHEF gel electrophoresisa | Conjugation | Probable location |
|---|---|---|---|---|---|
| pAM373-like region | |||||
| MMH594 | +b | C | Chromosome | ||
| V583 | + | NT | Chromosome | ||
| V586 | + | NT | Chromosome | ||
| 545 | + | NT | Chromosome | ||
| E99 | + | NT | Chromosome | ||
| NZ4 | + | NT | Chromosome | ||
| E19 | + | NT | Chromosome | ||
| E103 | + | NT | Chromosome | ||
| EV596 | + | NT | Chromosome | ||
| D6 | + | NT | Chromosome | ||
| EV638 | + | NT | Chromosome | ||
| 79-3 | + | NT | Chromosome | ||
| EV147 | + | NT | Chromosome | ||
| EV217-2 | + | NT | Chromosome | ||
| W32944 | − | E | Plasmid | ||
| E73 | − | E | Plasmid | ||
| EV108 | − | E | Plasmid | ||
| 613 | − | E | Plasmid | ||
| EV408 | − | E | Plasmid | ||
| E71 | − | E | Plasmid | ||
| Ned10 | − | E | Plasmid | ||
| Ned2 | − | E | Plasmid | ||
| EV419 | − | E | Plasmid | ||
| V105 | − | E | Plasmid | ||
| A-3-1 | − | E | Plasmid | ||
| NZ10 | − | E | Plasmid | ||
| A27 | − | E | Plasmid | ||
| 620 | − | E | Plasmid | ||
| 296 | − | E | Plasmid | ||
| A25 | − | E | Plasmid | ||
| EV447 | − | E | Plasmid | ||
| EV632 | − | E | Plasmid | ||
| NZ7 | − | E | Plasmid | ||
| EV321 | − | E | Plasmid | ||
| EV564 | − | E | Plasmid | ||
| OG1 | − | − | |||
| Cytolysin region | |||||
| MMH594 | + | +b | No | Chromosome | |
| V583 | − | +b | No | Chromosome | |
| V586 | − | +b | No | Chromosome | |
| 545 | − | + | No | Chromosome | |
| V105 | + | − | Yes | Plasmid | |
| Ned10 | + | − | Yes | Plasmid | |
| NZ4 | + | − | No | Chromosome | |
| E19 | + | + | Yes | Chromosome | |
| Ned2 | + | − | Yes | Plasmid | |
| D6 | + | + | No | Chromosome | |
| EV217-2 | + | − | Yes | Plasmid | |
| EV419 | + | − | Yes | Plasmid | |
| EV596 | + | − | Yes | Plasmid | |
| 296 | + | − | Yes | Plasmid | |
| W32944 | + | − | Yes | Plasmid | |
| E99 | + | + | No | Chromosome | |
| YI6-1 | + | −b | No | Chromosome |
a
See Materials and Methods. C, chromosomal; E, extrachromosomal; NT, not tested.
b
Previously characterized.