TABLE 1.
prhIR promoter activity in the wild type and the phcA mutant
| Plasmid | Promoter size (bp)a | β-Galactosidase activity (Miller units)b
|
|||
|---|---|---|---|---|---|
| OE1-1
|
RK5035c
|
||||
| Mean | Standard error | Mean | Standard error | ||
| pprhIR1 | 514 | 3,047 | 689 | 8,061 | 1,316 |
| pprhIR8 | 153 | 3,272 | 854 | 5,726 | 1,614 |
| pprhIR9 | 106 | 9,140 | 3,561 | 7,638 | 1,919 |
| pHRP309 | None | 1,429 | 310 | 1,482 | 361 |
a
Size refers to the region upstream from the start codon. The promoter region was fused to lacZ.
b
Cells were grown in BG medium up to a cell optical density at 600 nm of 1, and the enzyme activity was measured with SDS-chloroform-treated cells. Mean values and standard errors are in Miller units.
c
RK5035 is a phcA deletion mutant of OE1-1.