FIG. 6.

Inducibility of yusOP transcription by various antibiotics. Strain 168 was grown in L medium until the OD₆₅₀ reached 0.4, and antibiotics were added to final concentrations of 1 μg/ml for fusidic acid, 2 μg/ml for novobiocin, 10 μg/ml for streptomycin, and 0.2 μg/ml for actinomycin D. “None” represents no addition of antibiotics. Total RNAs were extracted from cells just before and after 30 min of treatment and used for RT-qPCR analysis. The transcription level was normalized relative to the amount of 16S rRNA in each RNA sample. The relative expression of yusP was calculated by dividing the relative amount of yusP in cells after treatment by that in cells before treatment. The average values (with standard deviations) from three independent experiments are shown. FA, fusidic acid; NB, novobiocin; SM, streptomycin; ACT, actinomycin D.