TABLE 2.
Oligonucleotides used in this study
| Primer | Oligonucleotide sequence (5′ → 3′)a |
|---|---|
| fusA1-F-seq | GGCTGAAGCAAACAAAGC |
| fusA1-R-seq | GATATCCCCTGCGTATACAG |
| fusA2-F-seq | GTTCTTCCGTGTGTACTCTG |
| fusA2-R-seq | TACCATGGTCAACGTGTC |
| yusO-F-seq | GATGTTCTTTCGGGCATC |
| yusO-R-seq | GTTGTCAGCCAGGTCATC |
| NyusO-F | AGCCTGAAATGCTGGAAAGC |
| NyusO-R | TCATCCGTT TCTGCTGCTTG |
| yusPH-F | CCCAAGCTTAGCCCTTGACGGTACC |
| yusPB-R | CGGGATCCCCGCAATAATTCCGACTG |
| PyusO-del-F | GTAAAATCAGTTGACTTTTCACTATTTTGTCATTAAAATATATATAC |
| PyusO-del-R | GTATATATATTTTAATGACAAAATAGTGAAAAGTCAACTGATTTTAC |
| PyusO-F1 | TACGATGTTCTTTCGGGCAT |
| PyusO-R1 | CACTCTTCATATTCATTTCCC |
| PyusO-F2 | TCTGTTGAAGCGGAGGATTC |
| PyusO-R2 | CTTGTTTCTCCATGCTTTCCAGC |
| yusON-F1 | CATATGAAGAGTGCGGATCAGTTAATG |
| yusOB-R1 | GGATCCTTATCCGTTTCCTCTTTTCATG |
| yusON-F2 | CATCATATGAAGAGTGCGGATCAGTT |
| yusOB-R2 | CGCGGATCCAATGGTACCGTCAAGGGCTG |
| RT-rrn16S-F | ATCTTCCGCAATGGACGA |
| RT-rrn16S-R | GCCGTGGCTTTCTGGTTA |
| RT-yusPF | TTCAGCCCTTGACGGTAC |
| RT-yusPR | TCCGCACAAAGCAGAAGC |
a
Underlining indicates sequences cleaved by the restriction enzymes HindIII, NdeI, and BamHI.