TABLE 2.

Oligonucleotides used in this study

Oligonucleotide	Sequencea
Oligonucleotides used to make polar mutations in trb genes
TDF	5′-GCTGGATCCATCGCTGGTGCGATGCTG-3′
TDR	5′-GCTTCTAGAACCATCTCGACCCCTTCAG-3′
TJF	5′-GCTGGATCCAATGGGCAATGTCGAAGATG-3′
TJR	5′-GCTTCTAGAGCGCGAGAATGACGATCAG-3′
TKF	5′-GCTTCTAGAGCAGGCACAAAAGGATCTG-3′
TKR	5′-CGGCGATACCGACCTCGATG-3′
TFF	5′-GCTGGATCCTCATCCCCTACATCGTTGAG-3′
TFR	5′-GCTTCTAGACCATGCCTTTCAAAGCTGTG-3′
Oligonucleotides used to subclone trb genes
TrbJKF-N	5′-GCTGAATTCGCAAAGGGGGATCGCCCATG-3′
TrbJR-N	5′-GTCGGATCCGAGAATGACGATCAGACGCG-3′
R10-trbKF-N	5′-GCTGAATTCGACGATGGAGCCACGCTGGTG-3′
R10-trbKJK3	5′-ATGAACATGATGCGTTTGAC-3′
Oligonucleotides used to clone Ptac-lacZ on pPZP200
MfeI-For	5′-GTCCAATTGTATACGCAAGGCGACAAGGTG-3′
NsiI-Rev	5′-GTCATGCATACTTATTCAGGCGTAGCACCA-3′
Oligonucleotides used for mutagenesis
pJ_W269Stop-F	5′-GAGCCACGCTGATGAGCTCGC-3′
pJ_W269Stop-R	5′-GCGAGCTCATCAGCGTGGCTC-3′
pK_W75Stop-F	5′-GAAACCGAGATGATAGTTCACC-3′
pK_W75Stop-R	5′-GGTGAACTATCATCTCGGTTTC-3′
pTacR1	5′-ACGACGTTGTAAAACGACGGC-3′
pTacR2	5′-GCCATTCAGGCTGCGCAACTG-3′
Oligonucleotides used for nuclease S1 protection assay
trbKS1	5′-GGCTGGACAGTAATCCAGGTGCCGATGCCTGCACTACGAC-3′
23SRNAS1	5′-AGGCTCGGGCTCCGACTGTTTGTAGGCATCCGGTTTCAG-3′

^aItalics indicate restriction endonuclease cleavage sites used in plasmid construction.