TABLE 5.
Expression of TrbJ and TrbK in recipients of multicopy plasmidsa
| pBBRMCS5 derivative (description or genotype) | pPZP200 derivative (description or genotype) | Conjugation efficiencyb (SD) | Exclusion coefficientc |
|---|---|---|---|
| pHC012 (vector) | pUP200 (vector) | 0.69 (0.2) | 1 |
| pJZ381 (traR)d | pPZP200 (vector) | 0.002 (0.001) | 345 |
| pUP402 (Ptac-trbJ) | pUP200 (vector) | 0.08 (0.009) | 8.6 |
| pHC012 (vector) | pUP403 (Ptac-trbJ) | 0.18 (0.03) | 3.8 |
| pHC368 (Ptac-trbK) | pUP200 (vector) | 0.10 (0.04) | 6.9 |
| pHC012 (vector) | pUP404 (Ptac-trbK) | 0.21 (0.04) | 3.3 |
| pUP402 (Ptac-trbJ) | pUP404 (Ptac-trbK) | 0.007 (0.002) | 98.6 |
| pHC368 (Ptac-trbK) | pUP403 (Ptac-trbJ) | 0.022 (0.004) | 31.4 |
| pHC361 (Ptac-trbJK) | pPZP200 (vector) | 0.002 (0.0008) | 345 |
a
The donor strain in each experiment was WCF5(pCF218), which overexpresses TraR and has an octopine-type Ti plasmid marked with a Kmr determinant. Transconjugants were selected using the Kmr gene of the Ti plasmid, the Spr gene of pUP200 or its derivatives, and the Gmr gene of pHC012 or its derivatives.
b
Number of transconjugants per donor.
c
The exclusion coefficient is the number of transconjugants per donor for the no-exclusion control (top line) divided by the number of transconjugants of the tested recipient strain per donor.
d
The overexpression of TraR by pJZ381 induces the expression of all tra and trb genes (6).