TABLE 6.
Effects of deleting the C-terminal Trp residues of TrbJ and TrbKa
| pBBRMCS5 derivative (description or genotype) | pPZP200 derivative (description or genotype) | Conjugation efficiencyb (SD) | Exclusion coefficient |
|---|---|---|---|
| pHC012 (vector) | pUP200 (vector) | 2.62 (0.88) | 1 |
| pHC012 (vector) | pUP403 (Ptac-trbJ) | 0.81 (0.68) | 3.2 |
| pHC012 (vector) | pUP408 (Ptac-trbJ*) | 1.66 (0.51) | 1.6 |
| pHC012 (vector) | pUP404 (Ptac-trbK) | 0.26 (0.06) | 10.0 |
| pHC012 (vector) | pUP406 (Ptac-trbK*) | 1.55 (0.69) | 1.7 |
| pUP402 (Ptac-trbJ) | pUP200 (vector) | 0.35 (0.23) | 7.4 |
| pUP407 (Ptac-trbJ*) | pUP200 (vector) | 0.63 (0.22) | 4.2 |
| pHC368 (Ptac-trbK) | pUP200 (vector) | 0.31 (0.14) | 8.5 |
| pUP405 (Ptac-trbK*) | pUP200 (vector) | 1.58 (0.68) | 1.7 |
| pUP402 (Ptac-trbJ) | pUP406 (Ptac-trbK*) | 0.17 (0.07) | 15.2 |
| pUP407 (Ptac-trbJ*) | pUP404 (Ptac-trbK) | 0.09 (0.04) | 28.4 |
| pHC368 (Ptac-trbK) | pUP408 (Ptac-trbJ*) | 0.21 (0.08) | 12.7 |
| pUP405 (Ptac-trbK*) | pUP403 (Ptac-trbJ) | 0.44 (0.12) | 5.9 |
| pUP407 (Ptac-trbJ*) | pUP406 (Ptac-trbK*) | 0.52 (0.26) | 5.1 |
| pUP405 (Ptac-trbK*) | pUP408 (Ptac-trbJ*) | 1.36 (0.07) | 1.9 |
| pUP402 (Ptac-trbJ) | pUP404 (Ptac-trbK) | 0.018 (0.008) | 143.9 |
| pHC368 (Ptac-trbK) | pUP403 (Ptac-trbJ) | 0.055 (0.018) | 47.6 |
a
The donor strain in each experiment was WCF5(pCF218), which overexpresses TraR and has an octopine-type Ti plasmid marked with a Kmr determinant. Transconjugants were selected using the Kmr gene of the Ti plasmid, the Spr gene of pUP200 or its derivatives, and the Gmr gene of pHC012 or its derivatives. The symbol * denotes a deletion of the last residue of the corresponding protein.
b
Number of transconjugants per donor.