. 2009 Mar 30;53(6):2522–2531. doi: 10.1128/AAC.00035-09

TABLE 1.

List of mutants hypersusceptible to at least two different antibiotics

PA14 gene locus	PAO1 ortholog	Gene name	Growth^b	Log₂ change in the MIC^a of:
PA14 gene locus	PAO1 ortholog	Gene name	Growth^b	SAM^c^,^d	ETP^c^,^d	CTX^c	CAZ^c	LVX^c	NAL^c^,^d	TGC^c^,^d	SXT^c
PA14_05530	PA0425	mexA	1.07	−1^f^,^g	−3^f^,^g	−1^f^,^g	≤−1^f^,^g	≤−1^g	−1^g	−2^f	≤−2
PA14_05540	PA0426	mexB	1.03	−2^f	−4^f	−2^f	≤−1^f	≤−1^g	−1^g		≤−2
PA14_07430	PA0572		1.05		−1^f^,^g	−1^f	≤−1^f	≤−1^g			−1
PA14_07770	PA0595	ostA	0.94				≤−1^f				−1
PA14_54410	PA0764	mucB	1.35			−1^f^,^g	≤−1^f^,^g	≤−1^g		−1
PA14_54330	PA0770^e	rnc	0.78				≤−1^f	≤−1		−1^f
PA14_53970	PA0794		1.19			−1^f	≤−1^f	≤−1^g			−1
PA14_53000	PA0871	phhB	0.66		−1^g		≤−1^f				≤−2
PA14_52260	PA0928		1.39			−1^f	≤−1^f	≤−1^g			−1
PA14_51780	PA0967	ruvB	1.15			−1^f	≤−1^f	≤−1			−1
PA14_51320	PA1005		1.01				≤−1^f	≤−1^g			−1
PA14_49320	PA1167		0.95		−1^g	−1^f	≤−1^f	≤−1^g			−1
PA14_47230	PA1316		1.01		−2^g	−1^f	≤−1^f				−1
PA14_41570	PA1777	oprF	1.17	−1^f^,^g	−2^f	−1^f^,^g	≤−1^f^,^g	≤−1^f^,^g		≤−4^f	≤−2
PA14_40620	PA1848		1.39			−1^f^,^g	≤−1^f^,^g	≤−1^g			−1
PA14_38530	PA2008	fahA	0.77	−1	−1^g	−1^f	≤−1^f
PA14_30290	PA2615^e	ftsK	1.31		−3^f	−2^f	≤−1^f	≤−1^f
PA14_27920	PA2800		1.18					≤−1^g		−2^f	−1
PA14_25110	PA3011	topA	0.88		−2^f^,^g	−1^f^,^g	≤−1^f^,^g			≤−4^f
PA14_23890	PA3110		0.89		−2^f^,^g	−1^f	≤−1^f
PA14_23360	PA3160	wzz	1.12				≤−1^f	≤−1^g		−1^f
PA14_22370	PA3233		1.07					≤−1^g		−2^f	−1
PA14_20730	PA3351	flgM	1.00		−1	−1^f^,^g	≤−1^f^,^g				−1
PA14_17170	PA3647^e	ompH	1.07		−1^f		≤−1^f	≤−1^f		−2	−1
PA14_16890	PA3670		0.92		−2	−1	≤−1	≤−1^g			−1
PA14_14910	PA3800		1.07			−1^f	≤−1^f	≤−1^f^,^g			−1
PA14_12400	PA3976	thiE	1.12			−1^f^,^g	≤−1^f^,^g	≤−1^g			−1
PA14_12030	PA4005		0.85		−2^g		≤−1^f
PA14_08780	PA4269	rpoC	0.96			−1^f^,^g	≤−1^f^,^g	≤−1^g		−1^f
PA14_57690	PA4441		1.00		−2^g	−1^f	≤−1^f	≤−1^g			−1
PA14_57880	PA4456		1.05					≤−1^g		−1	−1
PA14_57910	PA4459^e		1.31		−1		≤−1^f	≤−1		≤−4	−1
PA14_62560	PA4727	pcnB	1.09			−1^f	≤−1^f	≤−1^g		−2	−1
PA14_62770	PA4745^e	nusA	0.86		−3^g	−1^f	≤−1^f	≤−1^f		−1^f	−1
PA14_62880	PA4753		0.89		−2^g		≤−1^f
PA14_64190	PA4853^e	fis	1.06					≤−1		−1	−1
PA14_68670	PA5198		1.22		−2	−1	≤−1	≤−1
PA14_69810	PA5288	glnK	0.71		−2^g						−1
PA14_70980	PA5375	betT1	1.23			−1^f^,^g	≤−1^f^,^g	≤−1^f^,^g	−1^g		−1
PA14_15750	—^e		0.81		−1	−1^f	≤−1^f	≤−1		−2^f

Data are given only for those antibiotics for which a change in the MIC could be detected; only negative MIC changes are displayed.

Maximum growth rate of the mutant growth control relative to the growth of the wild type.

Retesting by agar dilution was performed using antibiotics of the same class: PIP for β-lactams (SAM), MEM for carbapenems (ETP), CAZ for cephalosporins (CAZ), CIP for quinolones (LVX, NAL), and TET for tetracyclines (TGC). SXT was not retested by agar dilution.

The MIC for the wild-type PA14 strain was above the calling range (Fig. 1); the MIC change was calculated based on an assumed MIC for the wild type of twice the upper limit of the calling range.

No PAO1 transposon mutant available. —, no PAO1 ortholog.

MIC change consistent with agar dilution testing of the PA14 mutant using an antibiotic of the same class.

MIC change consistent with agar dilution testing of the PAO1 mutant using an antibiotic of the same class.