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Genetic organization of the 13-kb region between the aac(6′)-Ib-cr and qnrS genes in the K. pneumoniae no. 135 clinical isolate. The genes and their transcription orientations are represented by horizontal arrows. For sequencing of plasmid DNA, PCR was done with primers F (5′-TTGCGATGCTCTATGAGTGGCTA-3′) and R (5′-TAAATTGGCACCCTGTAGGC-3′). Both strands of the PCR product were used for DNA sequencing.
