TABLE 2.
Primers used in this study
| Primer | Sequence (5′ to 3′) | Target |
|---|---|---|
| AmpC-2a | ACAGCCATACCTGGCACATC | blaampC |
| P13Bb | CGGGATCCCAGGCCCGGGAAC | 16S rRNA |
| UNI14 | GTGCCAGCAGCCGCGGTAAT | 16S rRNA |
| PBb | TAACACATGCAAGTCGAACG | 16S rRNA |
| BAK2 | GGACTACHAGGGTATCTAT | 16S rRNA |
| 16S1 | GGAGGAAGGTGGGGATGACG | RT-PCR of 16S rRNA gene |
| 16S3 | TCTGATCCGCGATTACTAGCG | RT-PCR of 16S rRNA gene |
| PreOXA-Ab-1 | CTAATAATTGATCTACTCAAG | blaOXA-51-like |
| PreOXA-Ab-2 | CCAGTGGATGGATAGATTATC | blaOXA-51-like |
| OXA-Abint-BamHI-A | CGGGATCCCGCGACCGAGTATGTACCTGCTT | blaOXA-66, internal primer |
| OXA-Abint-BamHI-B | CGGGATCCCGCTAAGTTAAGGGAGAACGC | blaOXA-66, internal primer |
a
The AmpC-2 primer is used with ISAba1-B.
b
The P13B primer is used together with UNI14; the PB primer is used together with BAK2.