FIG. 1.

Specific knockdown of B1R and G7L transcripts by siB1R-2 and siG7L-1 in A549 cells. Cells were transfected with siB1R-2, siG7L-1, or siNT (100 nM) and infected 24 h later with VACV-WR (MOI of 3). Real-time RT-PCR was performed as described in Materials and Methods. GAPDH was included as an endogenous reference and used for normalization. Error bars represent the standard deviations (SD) of the means of triplicates for at least two independent experiments. *, siRNAs differ significantly from the siNT control (Student's t test; P < 0.05). There was no significant difference between siNT-transfected infected cells and nontransfected infected cells.