FIG. 2.

Dose-dependent inhibition of VACV-WR replication by specific siRNAs in A549 cells. Various concentrations of siB1R-2 (A) and siG7L-1 (B) (1, 3, 10, 30, and 100 nM) or siNT (100 nM) were transfected into A549 cells. Twenty-four hours later, cells were infected with VACV-WR at an MOI of 0.1 and were harvested at 24 h postinfection. Viral titers were determined as described in Materials and Methods. Error bars represent the SD of the means of quadruplicates for at least two independent experiments. *, siB1R-2 or siG7L-1 differs significantly from the siNT control (Student's t test; P < 0.05). There was no significant difference between siNT-transfected infected cells and nontransfected infected cells.