FIG. 5.

Inhibition of MPXV by siB1R-2 and siG7L-1 in A549 cells. Cells were transfected with different concentrations (1, 3, 10, 30, or 100 nM) of siB1R-2 (A), siG7L-1 (B), or siNT (100 nM). At 24 hours posttransfection, cells were infected at an MOI of 0.1 with MPXV-Cop, and samples were harvested at 24 h postinfection. Viral titers were determined in cell culture. Error bars represent the SD of the means of quadruplicates for at least two independent experiments. *, P < 0.05 (siRNAs differ significantly from the siNT control) (Student's t test). There was no significant difference between siNT-transfected infected cells and nontransfected infected cells.