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. 2009 Mar 23;53(6):2579–2588. doi: 10.1128/AAC.01626-08

TABLE 2.

Antiviral activities of siRNAs in combination with cidofovir in A549 cellsa

siRNA Virus titer reduction (log10 TCID50/ml)b
Enhancement of cidofovir activitvc (log10 TCID50/ml) Synergistic interactiond (log10 TCID50/ml)
siRNA alone siRNA in combination with cidofovir
siB1R-2 0.5 1.1 +0.6 +0.1
siG7L-1 1.2 2.8 +2.3 +1.1
siD5R-2 1.2 3.0 +2.5 +1.3
0.5
a

Cells were transfected with the siRNA of interest (siD5R-2, siB1R-2, or siG7L-1) at a concentration of 1 nM. At 24 hours posttransfection, cells were infected with VACV-WR at an MOI of 0.0001 and then treated or not with cidofovir (32 μM). Samples were harvested at 48 h postinfection, and viral titers (log10 50% tissue culture infective dose [TCID50]/ml) were determined in cell culture.

b

Means of triplicate experiments.

c

Enhancement of cidofovir activity was calculated with the following formula: enhancement of cidofovir activity = (virus titer with combination of cidofovir and siRNA) − (virus titer with cidofovir alone).

d

The synergistic effect of the combination was calculated with the following formula: synergistic interaction = (viral titer with combination of cidofovir and siRNA) − (titer with siRNA alone + titer with cidofovir alone).