TABLE 2.
Overview of the MFs, mutation rates, and levels of resistance of the GO mutants
| Mutant | Doubling time (min) | Mutation rate | Mean MF in response to:
|
MIC (mg/liter) by Etesta (size of resistant mutant subpopulationb)
|
|||||
|---|---|---|---|---|---|---|---|---|---|
| Rifampin (300 μg/ml) | Streptomycin (500 μg/ml) | PIP | CAZ | TOB | CIP | ATM | |||
| PAO1 | 26 ± 0.6 | 6.75E-09 | 1.81E-8 | 8.48E-10 | 2 (+) | 2 | 1.5 | 0.19 | 1.5 |
| PAO1/pUCP26 | 1.37E-8 | 5.38E-10 | |||||||
| PAO1ΔmutY::Gm | 25.7 ± 1.7 | 3.85E-08 | 1.36E-7 | 3.51E-9 | 2 (++) | 1.5 (++) | 1.5 (+) | 0.19 (+) | 1 (+) |
| PAO1ΔmutY::Gm/pLM100 | 6.36E-8 | 1.29E-9 | |||||||
| PAO1ΔmutT::Gm | 27.7 ± 1.8 | 1.28E-07 | 5.07E-7 | 1.18E-7 | 2 (+++) | 2 (+++) | 1.5 (++) | 0.25 (++) | 1.5 (++) |
| PAO1ΔmutT::Gm/pLM101 | 3.91E-8 | 1.69E-9 | |||||||
| PAO1ΔmutM::Gm | 27.3 ± 1 | 6.38E-09 | 2.78E-8 | 1.69E-9 | 2 (++) | 1.5 (+) | 1.5 | 0.19 | 1.5 |
| PAO1ΔmutM::Gm/pLM102 | 2.53E-8 | 3.72E-10 | |||||||
a
PIP, piperacillin; CAZ, ceftazidime; TOB, tobramycin; CIP, ciprofloxacin; ATM, aztreonam.
b
The numbers of resistant mutant colonies in the inhibition zone are as follows: +, <10; ++, 10 to 100; +++, >100 (35).