TABLE 3.
MICs of various aminoglycosides for P. aeruginosa strains and E. coli strains transformed with aac(6′)-Iaf
| Straina | MICb (μg/ml) of:
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| AMK | ABK | DIB | GEM | ISP | KAN | NET | SIS | TOB | NEO | |
| P. aeruginosa IMCJ798 | 128 | 8 | >128 | 4 | >128 | >128 | >128 | 32 | 32 | 8 |
| P. aeruginosa IMCJ799 | 128 | 16 | >128 | 2 | >128 | >128 | >128 | 32 | 32 | 8 |
| E. coli JM109/pSTV28 | 1 | 2 | 1 | 0.5 | 1 | 2 | 0.5 | 0.5 | 0.125 | 2 |
| E. coli JM109/pSTV-aacWT | 16 | 4 | 16 | 1 | 4 | 64 | 8 | 1 | 4 | 4 |
| E. coli JM109/pSTV-aac(TTG→ATG) | 32 | 4 | 32 | 1 | 16 | 128 | 8 | 2 | 4 | 4 |
a
The MICs for E. coli strains were determined with Mueller-Hinton broth preparations containing chloramphenicol (30 μg/ml) and individual aminoglycosides.
b
AMK, amikacin; ABK, arbekacin; DIB, dibekacin; GEM, gentamicin; ISP, isopamicin; KAN, kanamycin; NET, netilmicin; SIS, sisomicin; TOB, tobramycin; NEO, neomycin.