TABLE 3.
Phenotypic and genotypic characteristics of C. difficile mutants obtained after selection by LE
| Parent strain | Mutants selected | Step of selection (μg/ml LE) | MIC (μg/ml)
|
Substitution(s) in:
|
|||
|---|---|---|---|---|---|---|---|
| Agar dilution method for:
|
Etest for GA | ||||||
| LE | MX | GyrA | GyrB | ||||
| C253 | C253 A, B, C | I (4) | 4 | 1 | 0.5 | ||
| C253 D, E, F | II (8) | 8 | 2 | 1 | |||
| III (16) | |||||||
| CD5 | CD5 A, B, C | I (4) | 4 | 1 | 0.5 | Ser416 to Ala | |
| CD5 D, E, F | II (8) | 8 | 2 | 2 | Ser416 to Ala | ||
| III (16) | |||||||
| A422 | A422 A, B, C | I (4) | 4 | 1 | 1 | ||
| A422 D, E, F | II (8) | 8 | 2 | 2 | Leu451 to Phe | ||
| A422 G, H, I | III (16) | 256 | 64 | ≥32 | Thr82 to Ile | Leu451 to Phe | |
| 630 | 630 A, B, C | I (4) | 4 | 1 | 0.75 | ||
| 630 D, E, F | II (8) | 32 | 8 | 3 | Ala118 to Val | ||
| BI2 | BI2 A, B, C | I (4) | 4 | 1 | 2 | ||
| BI2 D, E, F | II (8) | 64 | 16 | ≥32 | Glu466 to Lys | ||