TABLE 1.

Primers used in this work

Primer	Sequence (5′-3′)	Positionb	Usage of primer pair
P1a	AAAGAATAAAATGAAAACAGT	1855	PCR of cfiA, probe for Southern hybridization, detection of protein polymorphisms
P4a	GATAAAAGTTTTCGCCTCTTC	2260
P5	AAGCAGGGATCTGCTTAC	164	PCR of orf1-orf3 intergenic sequence: detection of sequence polymorphisms
P6	ATGACCGTATTCAGATAG	1109
R1	TGCGACAGGGAAAAGCATGGAGA	1867	iPCR of cfiA upstream region: detection of sequence polymorphisms.
R2	CCCTCGCCGAAATCGAAGGATG	1990

^aDescribed in reference 2.

^bThe 3′ ends of primers are positioned in relation to the sequence under accession no. FM200792.