FIG. 1.

Influence of omeprazole (Omep) on the growth yield of S. Typhimurium 14028 bacteria in RAW264.7 cells. The increase in intracellular bacteria between 2 and 16 h postinfection was determined by viable counting, and the ratio is displayed as the growth yield (A) or by enumerating the mean number of intracellular bacteria per microscopic field at 16 h postinfection (B). Representative microscopic fields for untreated (C) and omeprazole-treated (D) infected cells are shown. Nuclei of RAW264.7 cells have been stained with 4′,6′-diamidino-2-phenylindole (DAPI) (blue), and the bacteria are revealed as green through the expression of GFP. For each sample, 20 random microscopy fields were selected and processed for statistic analyses. The arrows indicate the perinuclear localization of bacteria. The statistical difference between the growth yields was confirmed in a paired student's t test (***, P < 0.0005).