FIG. 3.

Proton pump inhibitors differently affect the viability of intracellular S. Typhimurium. (A) Bafilomycin A₁ had a stronger inhibitory effect on the intracellular replication of S. Typhimurium in RAW264.7 cells. (B) RAW264.7 cells infected with S. Typhimurium 14028 were treated simultaneously with tetracycline (10 μg/ml) to cause a reversible inhibition of bacterial protein synthesis and replication, in conjunction with either omeprazole (Omep) or bafilomycin A₁ (Baf). This analysis showed that tetracycline completely inhibited bacterial intracellular replication, and that there was no additive effect by combining omeprazole and tetracycline (Tet). In contrast, bafilomycin A₁ caused a significant decrease in the numbers of tetracycline-treated bacteria. The statistical difference between the growth yields was confirmed in a paired student's t test (*, P < 0.05).