. 2009 Apr 10;75(11):3477–3483. doi: 10.1128/AEM.00285-09

TABLE 3.

Sensitivity of three real-time PCR assays in the detection of T. gondii oocysts seeded into water sample concentrates

Estimated no. of oocyst/ 0.5 ml water concentrate	B1-Burg PCR^a			B1-Costa-PCR^b			529 PCR^c			P^d
	Positive rate	C_T		Positive rate	C_T		Positive rate	C_T
	Positive rate	Mean	SD	Positive rate	Mean	SD	Positive rate	Mean	SD
1,600	6/6	28.17	0.41	6/6	29.67	0.52	6/6	24.67	0.82	1.000
800	6/6	30.33	0.82	6/6	30.67	0.82	6/6	26.17	0.75	1.000
400	6/6	32.17	1.60	6/6	31.50	1.05	6/6	27.33	0.52	1.000
200	3/6	32.00	0.00	6/6	32.33	0.82	6/6	28.33	0.82	0.027
100	3/6	34.67	1.15	4/6	32.50	0.58	6/6	29.33	0.82	0.144
50	0/6			1/6	34.00		6/6	30.50	1.05	0.004
25	1/6	36.00		0/6			6/6	30.83	0.98	0.001
10	0/6			0/6			6/6	32.17	1.33	0.0001
5	0/6			0/6			2/6	31.50	0.71	0.1054
1	1/6	36.00		1/6	33.00		3/6	32.33	2.31	0.330
0	0/6			0/6			0/6			1.000

Real-time PCR based on B1 primers by Burg et al. (6) and FRET probes designed in this study.

Real-time PCR based on the B1 gene by Costa et al. (12).

Real-time PCR based on the 529-bp repetitive element by Reischl et al. (45).

P values for differences in detection rates among the three PCR assays were determined by chi-square analysis.