TABLE 4.
Sensitivity (CT values) of 529 PCR using the Roche ready-to-use buffer (master hybridization probes) and in-house PCR mix in the detection of T. gondii oocysts seeded into storm water concentratesa
| Estimated no. of oocyst/ 0.5 ml water concentrate | Master hybridization probe kit (Roche)
|
In-house mixture
|
P | ||||
|---|---|---|---|---|---|---|---|
| Positive rate |
CT
|
Positive rate |
CT
|
||||
| Mean | SD | Mean | SD | ||||
| 1,600 | 4/4 | 22.75 | 4.27 | 4/4 | 24.75 | 0.96 | 1.000 |
| 800 | 4/4 | 25.25 | 4.57 | 4/4 | 26.25 | 0.96 | 1.000 |
| 400 | 4/4 | 26.00 | 4.00 | 4/4 | 27.25 | 0.50 | 1.000 |
| 200 | 4/4 | 27.75 | 2.87 | 4/4 | 28.25 | 0.96 | 1.000 |
| 100 | 4/4 | 33.25 | 9.84 | 4/4 | 29.25 | 0.96 | 1.000 |
| 50 | 3/4 | 30.00 | 0.00 | 4/4 | 30.25 | 0.96 | 0.500 |
| 25 | 4/4 | 35.00 | 10.03 | 4/4 | 30.75 | 0.96 | 1.000 |
| 10 | 3/4 | 31.33 | 1.15 | 4/4 | 32.25 | 1.71 | 0.500 |
| 5 | 3/4 | 34.67 | 2.31 | 2/4 | 31.50 | 0.71 | 0.500 |
| 1 | 1/4 | 33.00 | — | 2/4 | 33.00 | 2.83 | 0.500 |
| 0 | 0/4 | — | — | 0/4 | — | — | 1.000 |
a
Dashes indicate no amplification. P values for differences were determined by chi-square analysis.