TABLE 3.
Result of human-associated markers PCR assays on nontarget and target samplesa
| Sample | Description | Source | No. of samples tested | No. of samplesb detected by:
|
||||
|---|---|---|---|---|---|---|---|---|
| Total Bacteroidetes PCR | HPyV QPCR | Human-associated Bacteroidetes PCR | M. smithii PCR | Adenovirus PCR | ||||
| Nontarget DNA virus | Adenovirus | ATCC VR-846 | 1 | ND | 0 | ND | ND | 1 |
| Target viruses | JCV | ATCC VR-1583 | 1 | ND | 1 | ND | ND | ND |
| BKV | ATCC VR-837 | 1 | ND | 1 | ND | ND | ND | |
| Animal fecal samples | Cat (Felis catus) | Individuals | 5 | 5 | 0 | 1 | 0 | ND |
| Chicken (Gallus gallus) | Individuals | 1 | 1 | 0 | 0 | 0 | ND | |
| Cow (Bos taurus) | Individuals | 24 | 24 | 0 | 0 | 1 | ND | |
| Sandhill crane (Grus canadensis) | Individuals | 2 | 2 | 0 | 0 | 0 | ND | |
| Deer (Odocoileus virginianus) | Individuals | 3 | 3 | 0 | 0 | 0 | ND | |
| Dog (Canis lupus familiaris) | Individuals | 55 | 55 | 0 | 14 | 0 | ND | |
| Duck (Anas platyrhynchos) | Individuals | 4 | 4 | 0 | 0 | 0 | ND | |
| Fox (Vulpes vulpes) | Individuals | 1 | 1 | 0 | 0 | 0 | ND | |
| Horse (Equus caballus) | Individuals | 8 | 8 | 0 | 0 | 0 | ND | |
| Racoon (Procyon lotor) | Individuals | 1 | 1 | 0 | 0 | 0 | ND | |
| Seagull (Larus atricilla) | Individuals | 6 | 6 | 0 | 0 | 0 | ND | |
| Sparrow (Ammodramus savannarum) | Individuals | 3 | 3 | 0 | 0 | 0 | ND | |
| Wild pig (Sus scrofa) | Individuals | 2 | 2 | 0 | 0 | 0 | ND | |
| Animal composite waste samples | Cow (Bos taurus) | Farm composite | 1 | 1 | 0 | 0 | 0 | ND |
| Pig (Sus scrofa domestica) | Farm composite | 1 | 1 | 0 | 0 | 0 | ND | |
| Animal urine samples | Dog (Canis lupus familiaris) | Individuals | 9 | ND | 0 | ND | ND | ND |
| Cat (Felis catus) | Composite of three cats | 1 | ND | 0 | ND | ND | ND | |
| Target human samples | Lift station | Composite | 2 | ND | 2 | 2 | 2 | 2 |
| Sewage influent | Composite | 39 | ND | 39 | 39 | 39 | 39 | |
| Septic tank pump truck | Composite of two to three septic tanks | 9 | ND | 9 | 9 | 9 | 7 | |
| Dechlorinated tertiary-treated WWTP effluent | Effluent | 9 | ND | 2 | 0 | 0 | 1 | |
| Septic tanks | Composite of two to four individuals | 5 | ND | 5 | 3 | 4 | 0 | |
| Urine samples | Individuals | 26 | ND | 6 | ND | ND | ND | |
a
Boldface values indicate amplification in nontarget samples.
b
That is, the number of samples quantifiable by QPCR or amplified by conventional PCR. ND, the sample(s) was not analyzed by the indicated assay,