Stx1Ab titers. Titers in dam sera (A) and colostrums (B) and in calf sera taken after colostrum uptake, within the first 24 h of age (C), were quantified by VNA. n.t., sample not tested. Dam 15 is listed twice because this dam delivered twin calves (15a and 15b). (D) Dynamics of Stx1Ab titers in calf sera until the 24th week of age. Charts for selected individual calves are depicted in color. Calves 3, 5, 13, 17, and 20 fulfilled the criteria for Stx1-specific seroconversion, as defined in Materials and Methods. Values represent relative mean values from triplicate determinations in single VNA experiments; SD were smaller than 6% of the corresponding mean values and were omitted from the figure.